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For the last-month rule, the testing period begins with the last month of your tax year and ends on the last day of the 12th month following that month (for example, December 1, 2019, through December 31, 2020)

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This structural transition leads to a separation of the ICD, at least to the Box1Box2 motif, and dissociates the JAK2 kinase-pseudokinase trans interaction and brings the KDs in proximity allowing trans phosphorylation and activation (1, 51)

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This cross-reactivity represents a significant limitation hindering the accurate and specific quantification of mBDNF using immunoassays